Patent Number / Registration Number: 1-2013-000158
Status of Application: On – going
Brief Description / Features of the Technology:
Loop-mediated isothermal amplification (LAMP) is a new molecular technique available, which can equal the PCR in specificity and offer higher sensitivity and rapid detection under isothermal conditions.
The sensitivity of the reaction is enhanced by the use of four to six specially designed primers to detect white spot syndrome virus (WSSV). LAMP is ten times more sensitive for the detection of WSSV than conventional PCR. The use of four to six primers, instead of the usual two primers in PCR, also speeds up the reaction thereby reducing detection time. Results can therefore be obtained within one hour.
Advantages:
Because of its convenience, simplicity, and speed of detection, LAMP can therefore be used for on-site detection of WSSV in shrimp. Moreover, the practicality of LAMP assay makes it an effective tool for routine diagnosis for early detection of WSSV or any pathogen so proper health management procedures can be implemented to prevent mortality and avert economic losses.
Target Beneficiaries: Shrimp growers.
Contact Details:
Dr. Mary Beth B. Maningas
University of Santo Tomas
(02) 732 7486